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The mission of BioCARS is to provide state-of-the-art X-ray facility, scientific and technical expertise and support to enable users to study the dynamic properties of biological macromolecules by X-ray scattering techniques: time-resolved diffraction and solution scattering (SAXS/WAXS). In hybrid mode of the APS storage ring, BioCARS 14-ID beamline provides high polychromatic flux, with ~3 × 1010 photons per 100ps pulse delivered to the sample. Short X-ray pulses are synchronized with ps or ns laser pulses for conducting pump-probe time-resolved experiments. In recent years we developed and implemented these technologies that are now offered as standard methods to BioCARS users:
- Serial Laue micro-crystallography using fixed targets and crystal injectors to facilitate studies of irreversible reactions while minimizing sample consumption.
- Electric-field jump as a method for reaction initiation and studies of protein dynamics.
- Novel temperature-controlled sample cells for temperature-jump solution scattering studies.
- Sample-minimizing co-flow cell for time-resolved solution scattering studies of irreversible reactions.
The overall goal of time-resolved experiments at BioCARS is to understand basic biological processes in structural and dynamics terms, on time scales from 100 picoseconds to seconds.
Use of BioCARS is supported by the National Institute of General Medical Sciences of the National Institutes of Health under grant number P41 GM118217. Funding acknowledgement for publications resulting from use of BioCARS facility can be found here.
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Chan, A. M., Nijhawan, A. K., Hsu, D. J., Leshchev, D., Rimmerman, D., Kosheleva, I., Kohlstedt, K. L., and Chen, L. X.
The Role of Transient Intermediate Structures in the Unfolding of the Trp-Cage Fast-Folding Protein: Generating Ensembles from Time-Resolved X-ray Solution Scattering with Genetic Algorithms.
J. Phys. Chem. Lett. 14, 1133–1139 (2023).
Dalton, K. M., Greisman, J. B., and Hekstra, D. R.
A unifying Bayesian framework for merging X-ray diffraction data.
Nat Commun 13, 7764 (2022).
Wilamowski, M., Sherrell, D. A., Kim, Y., Lavens, A., Henning, R. W., Lazarski, K., Shigemoto, A., Endres, M., Maltseva, N., Babnigg, G., Burdette, S. C., Srajer, V., and Joachimiak, A.
Time-resolved β-lactam cleavage by L1 metallo-β-lactamase.
Nat Commun 13, 7379 (2022).
Lee, S. J., Kim, T. W., Kim, J. G., Yang, C., Yun, S. R., Kim, C., Ren, Z., Kumarapperuma, I., Kuk, J., Moffat, K., Yang, X., and Ihee, H. (2022)
Light-induced protein structural dynamics in bacteriophytochrome revealed by time-resolved x-ray solution scattering.
Science Advances 8, eabm6278.
Moreno-Chicano et al. (2022)
Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature.
IUCrJ 9, 610–624.
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Wilamowski, M., Sherrell, D. A., Kim, Y., Lavens, A., Henning, R. W., Lazarski, K., Shigemoto, A., Endres, M., Maltseva, N., Babnigg, G., Burdette, S. C., Srajer, V., and Joachimiak, A. (2022) Time-resolved β-lactam cleavage by L1 metallo-β-lactamase. Nat Commun 13, 7379.
CARS is proud to announce Dr. Peter Eng has been appointed the new CARS Executive Director, effective September 1, 2022. Our sincere thanks goes to Dr. Mark Rivers for his nine years of service and leadership to CARS.
Eric will be the new Beamline Mechanical Technician for Sector 14.
Light-Induced Protein Structural Dynamics in Bacteriophytochrome Revealed by Time-Resolved X-ray Solution Scattering
Lee, S. J., Kim, T. W., Kim, J. G., Yang, C., Yun, S. R., Kim, C., Ren, Z., Kumarapperuma, I., Kuk, J., Moffat, K., Yang, X., and Ihee, H. (2022) Light-induced protein structural dynamics in bacteriophytochrome revealed by time-resolved x-ray solution scattering. Science Advances 8, eabm6278.
Biju, L. M., Wang, C., Kang, W., Tom, I. P., Kumarapperuma, I., Yang, X., and Ren, Z. (2022) On-chip Crystallization and Large-Scale Serial Diffraction at Room Temperature. JoVE (Journal of Visualized Experiments) e63022.
Cho, H. S., Schotte, F., Stadnytskyi, V., and Anfinrud, P. (2021) Time-resolved X-ray scattering studies of proteins.
Current Opinion in Structural Biology 70, 99–107.
BioCARS was pleased to provide a tour and discuss science conducted at our facility with young women who participated in the Science Careers in Search of Women Program.
Kosheleva, I., Henning, R., Kim, I., Kim, S. O., Kusel, M., and Srajer, V.